Liquid Chromatography-Mass Spectrometry is one of the most precious tools. Its high sensitivity and selectivity has enhanced its value for being used in several industries. It is well-suited for carrying out analysis of large, ionic, polar, non-volatile and thermally unstable compounds. Spectro offers a wide platform to get training on this valuable instrument. A mass spectrometer combined with a liquid chromatograph can detect masses characteristic of a compound. A complete detailed description of the instruments, along with its theory and technique to use their accessories is given.