Analytical Instruments Testing Series-Training on HPLC

An outstanding platform is being offered by Spectro to get an exposure to High Performance Liquid Chromatography technique. A complete theoretical as well as practical knowledge on HPLC, its maintenance, method development and troubleshooting techniques can be extracted from this training. Valuable information about the modernized HPLC components, its pumps and detectors is imparted.

Aim of the Program:

Spectro offers a beneficial understanding and hands-on practice on Agilent HPLC Binary-1200 Series, 1200 Infinite series, HPLC Binary with Fluorescence detector (RF 10 AXL, Shimadzu) and HPLC Isocratic with UV-VIS detector (LC-10 ATVPS, Shimadzu) that will help the trainee to become a part of various industries. The training intensifies the knowledge of a trainee to make him capable for applying modern practices of HPLC. The awareness for making a selection of relevant instrumentation and columns, interpreting and troubleshooting the chromatograms is imparted.

Minimum Eligibility Required The trainee must hold a Diploma or Graduation Degree in Science field or must be pursuing the same.

Training Module for 6 Days

Day 1
Introduction to basic principles of chromatography, several HPLC detectors with their applications, columns, binary and isocratic gradients, mobile phases, polarity and retention time.
Day 2
Function of UV-Vis detectors and Fluorescence detectors, their working, calibration and applications.
Day 3
Preparing test solutions, Mobile Phase, Setup method for HPLC, Data acquisition software.
Day 4
Software Quantization, Integration and Report Generation.
Day 5
Method development, optimization of various chromatographic parameters.
Day 6
Understanding specific applications, prevention, care, maintenance and trouble shooting.

Advantages of the Training Program:

The training is more profitable for the person who wants to make his career in various industries which works with chemicals, cosmetics, oils, agriculture, food and analytical testing laboratories, etc. Training on HPLC will open the gates of such industries as it is the most indispensable instrument which is used in drugs and pharmaceuticals.

Please Note:


  • The certificate will be provided only after clearing a test
  • We also conduct customized training programs on request.